تسجيل الدخول
CRYO TECHNIQUES [Freeze Fracture and Freeze Etching Equipment, JFD-9010]

The financial grant for this lab is given by KFAS. This lab is equipped with JFD-9010 freeze etching and other supporting equipment for sample preparation.

The JFD-9010 freeze etching equipment is used to prepare specimens for electron microscopy by various treatments of fresh biological specimens frozen by rapid freezing. The treatments include freeze fracturing and freeze-etching in high vacuum for maintaining cells and tissues of such specimens in an almost native state, plus electron beam evaporation for making replica films of ultra-micro particles.

Also, use of electron beam evaporator and specimen rotation/tilt mechanism allows low-angle rotational evaporation to be performed with ease. This is extremely useful for molecular biologists who employ electron microscope for high-resolution observation of protein molecules.

1. Sectioning down to -185 ° C.

2. New contact-free through-the-wall specimen holder system for chatter free cryosection.

3. Open top design with no disturbing ice-condensation inside or outside during or after work.

4. Attaches to the Ultracut S and is ready for sectioning at low temperature within a few minutes.

5. Memory for 3x3 settings of knife specimen and gas temperature.

6. Continuous turbulence-free no-pressure LN2 filling system with a very low LN2 consumption.

7. New shell mounted sectioning chamber with armrest for comfortable manipulation of sections.

8. Accurate control of specimen, knife and the most important chamber gas temperature.

9. Backlight illumination for accurate glass and safe diamond knife approach.

10. Alignment of knife with self-locking drives from outside the chamber.

This system with Cryo holder, ultra thin sections grids can be loaded directly from Cryoultramicrotome to the cryo holder in the microtomy room. Then it can be transferred at low temperature to the Transmission electron microscope.